Species identification of some non-domestic animals using PCR-RFLP analysis of cytochrome b gene [electronic resource].

Includes references.

PCR and PCR-RFLP methods were used to discriminate between some non domestic animals (american black bear, blue nile monkey, Barbary sheep, bacterian camel and llama) and human. DNA from hair was extracted to amplify a mitochondrial cytochrome b gene segment (358 bp) using universal primer followed by agarose gel electrophoresis. The total cyt b amplicon was digested with 4 restriction endonucleases (Alu I , Hae III, HinfI and Taq I ) and the resulting fragments were resolved through electrophoresis. The different specific electrophoretic patterns and total restriction fragments were clearly observed among the studied species.

Summary in Arabic.

1