Zoonotic and microbiological aspects of escherichia coli 0:157 with special reference to its RAPD-PCR [electronic resource].

Includes references.

A total of 160 animals, water, food and human samples were collected and examined for the presence of Escherichia coli 0: 157. The samples included 53 fecal swab of farm animals (19 sheep, 18 buffaloes, 16 cattle), 21 water (6 wells, 7 public and 8 River Nile), 46 food (14 raw milk, 12 chicken meat and 20 crayfish, Procambarus clarkii ), 20 surgically excised appendices and 20 human fecal swab samples. E. coli 0: 157 was detected in 3 out of 18 (16.7%) buffalo fecal samples. meanwhile, E. coli 0:157 was neither detected from any examined cattle nor sheep fecal samples. Only one River Nile water sample out of 8 (12.5%) was positive for E. coli 0:157, but the well and public water samples were free from, it. Moreover, two isolates were recovered from raw milk and chicken meat samples (one isolate, each) with the percentages of 7.1 % and 8.3% respectively. On the other hand, E. coli 0: 157 was not detected from any examined crayfish samples. Regarding human samples, one isolate from an appendix and 2 from human stool were identified with the percentage of 5% and 10%, respectively.

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