Detection of eaeA and hlyA genes in escherichia coli-causing diarrhoea in young calves by multiplex PCR [electronic resource].

Includes references.

E.coli was isolated from the faeces of 28/31 (90.3%) diarrhoeic and 9/11 (81.8%) apparently heallhy calves. E. coli O157 was the most prevalent serotype, detected from 5/31 diarrhoeic calves (16.1 %). No 0157 E. coli was revealed from the apparently healthy calves. Other serotypes were detected in diarrhoeic calves as 0136, 0111, 0118, 0103, 091, 0121, 0145, 087, 022 and 08 whereas 091, 0113, 087, and 08 were revealed from the apparently healthy. There was also 3 untypable E. coli isolates. Screening virulence genes eaeA and hlyA in identified isolates or in faecal culture crude extract revealed clear PCR products, eaeA gene of 384bp and hlyA of 534bp. Isolates from diarrhoeic calves possesed 20/29 (69%) eaeA gene and 21/29 (72.4%) hlyA gene.

1