Effect of glutathione on the freezability and in vitro fertilizing potentials of bovine spermatozoa [electronic resource].

Includes references.

Cryopreservation induces sub lethal damage to the spermatozoa, thereby reduce their fertile life. In the present study, effect of glutathione on bovine semen freezability and in vitro fertilizing potentials were evaluated. Semen was cryopreserved in tris-based extender supplemented with different concentrations of reduced glutathione (0, 0.5, 1,2,3 and 5 mM). Semen post-thawing motility, viability and acrosomal integrity, DNA damage, enzymes leakage, total antioxidant capacity (TAC), lipid peroxidation and in vitro fertilizing potentials were assessed. Current results indicated that addition of 2 mM glutathione to semen extender significantly (P<O.D5) improved post-thawing motility, viability and acrosomal integrity (66.25±5.54%, 169.38±18.59 and 12.75±3.45%, respectively).

Summary in Arabic.