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Isolation and identification of some PPFMs bacterial isolates and their potentiality as biofertilizers and biocontrol agents to Rhizoctonia solani [electronic resource].

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Kassem, M. M

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Language: English Summary language: Arabic Description: p.79-92Other title:

عزل وتعريف لبعض عزلات PPFMs البكتيرية وكفاءتها كمسمدات حيوية ومضادات حيوية لفطر الريزوكتونيا سولانى [Added title page title]

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Mansoura University journal of agricultural chemistry and biotechnology, 2013 v.4 (2) [electronic resource].

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In: Mansoura University Journal of Agricultural chemistry and biotechnology 2013.v.4(2)Summary: A total of 12 isolates of pink-pigmented, facultative methylotrophic bacteria (PPFMs) were isolated on selective AMS medium from clay soil. Samples were collected from two locations (Sidi Salem and Agricultural Farm, Sakha Agri. Res. Station). There after. all the classical recommended methods used for identification of all the obtained isolates were carried out including cultural, morphological, microscopical as well as biochemical characteristics. The results revealed that all the isolates were gram negative, rod shaped, motile, colonies were light pink or pink or orange/pink in colour due to pigmentation. Biochemical characteristics revealed that all the isolates showed positive results for oxidase, catalase, urease and citirate test as well as indole production. None of the isolates showed positive results for casein hydrolysis. The isolates of ML₄, ML₈ and ML₁₀ gave a positive reaction for starch hydrolysis test, while the others showed negative results. Regarding, cellulose degradation, the isolates ML₂, ML₇,ML₈ and ML₁₂ gave a positive reaction. The obtained results highly recommended that all the obtained isolates could be classified as follows: ML₈ and ML₆ Methylobacterium radiotolerans; ML₂ and ML₅ Methylobacterium suomiense; ML₃ Methylobacterium aminovorans; ML₄ and ML₉ Methylobacterium thiocyanatum; ML₇, ML₈ and ML₁₀ Methylobacterium rhodesianum; ML₁₁ Methylobacterium fujisawaens and ML₁₂ Methylobacterium rhodinum. Screening the isolates for beneficial growth characters and biocontrol agent to R. solani. In general, Methylobacterium rhodinum.and Methylobacterium aminovorans recorded the highest values of indole acetic acid production, 28.15 and 27.50 µg ml⁻¹, and 70.41 and 67.46 µg ml⁻¹ for GA production, and 0.580, 0.560 µ moles of siderophores production, and 1.217 and 1.193 mg N/g for fixed nitrogen respectively.

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A total of 12 isolates of pink-pigmented, facultative methylotrophic bacteria (PPFMs) were isolated on selective AMS medium from clay soil. Samples were collected from two locations (Sidi Salem and Agricultural Farm, Sakha Agri. Res. Station). There after. all the classical recommended methods used for identification of all the obtained isolates were carried out including cultural, morphological, microscopical as well as biochemical characteristics. The results revealed that all the isolates were gram negative, rod shaped, motile, colonies were light pink or pink or orange/pink in colour due to pigmentation. Biochemical characteristics revealed that all the isolates showed positive results for oxidase, catalase, urease and citirate test as well as indole production. None of the isolates showed positive results for casein hydrolysis. The isolates of ML₄, ML₈ and ML₁₀ gave a positive reaction for starch hydrolysis test, while the others showed negative results. Regarding, cellulose degradation, the isolates ML₂, ML₇,ML₈ and ML₁₂ gave a positive reaction. The obtained results highly recommended that all the obtained isolates could be classified as follows: ML₈ and ML₆ Methylobacterium radiotolerans; ML₂ and ML₅ Methylobacterium suomiense; ML₃ Methylobacterium aminovorans; ML₄ and ML₉ Methylobacterium thiocyanatum; ML₇, ML₈ and ML₁₀ Methylobacterium rhodesianum; ML₁₁ Methylobacterium fujisawaens and ML₁₂ Methylobacterium rhodinum. Screening the isolates for beneficial growth characters and biocontrol agent to R. solani. In general, Methylobacterium rhodinum.and Methylobacterium aminovorans recorded the highest values of indole acetic acid production, 28.15 and 27.50 µg ml⁻¹, and 70.41 and 67.46 µg ml⁻¹ for GA production, and 0.580, 0.560 µ moles of siderophores production, and 1.217 and 1.193 mg N/g for fixed nitrogen respectively.

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