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Cytotoxic effects of entomopathogenic fungal crude filtrate on Phthorimaea operculella cell line [electronic resource].

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Khamiss, O

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Language: English Summary language: Arabic Description: p.141-148Other title:

التأثيرات الخلوية السامة لراشح بعض الفطريات الممرضة للحشرات علي مزارع الخلايا من فراشات درنات البطاطس [Added title page title]

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Arab journal of biotechnology, 2001 v. 4 (2) [electronic resource]:

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In: Arab Journal of Biotechnology 2001.v.4(2)Summary: A Phthorimaea operculella cell line was used to study the cytotoxic effects of the entomopathogenic fungi Metahrizium anisopliae, Beauveria bassiana and Verticillium lecanii. P. operculella. Cell treatment with fungi crude filtrate (in shake culture and solid medium) showed a clear cellular cytopathic effect. Cells treated with M.anisopliae crude filtrate revealed granular contents, irregular forms and alterations on the cellular membrane. Treated cells of P.operculella with B. bassiana and V. lecanii crude filtrates showed elongation in cell shape. P.opercullela treated cells with sonicated conidospores filtrate of each of the three fungi isolates showed irregular forms and intensive vacuoles. However, treated cells with filtrated and then sonicated blastosopres of the fungi showed associated crystals in the culture medium and fibroplast elongated cells filled with intensive vacuoles. This study suggests that the sonicated blastospores induce more obvious cellular changes and higher toxic effect than crude broth filtrate secreted in fungi media.

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A Phthorimaea operculella cell line was used to study the cytotoxic effects of the entomopathogenic fungi Metahrizium anisopliae, Beauveria bassiana and Verticillium lecanii. P. operculella. Cell treatment with fungi crude filtrate (in shake culture and solid medium) showed a clear cellular cytopathic effect. Cells treated with M.anisopliae crude filtrate revealed granular contents, irregular forms and alterations on the cellular membrane. Treated cells of P.operculella with B. bassiana and V. lecanii crude filtrates showed elongation in cell shape. P.opercullela treated cells with sonicated conidospores filtrate of each of the three fungi isolates showed irregular forms and intensive vacuoles. However, treated cells with filtrated and then sonicated blastosopres of the fungi showed associated crystals in the culture medium and fibroplast elongated cells filled with intensive vacuoles. This study suggests that the sonicated blastospores induce more obvious cellular changes and higher toxic effect than crude broth filtrate secreted in fungi media.

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