Biochemical genetic discrimination of some selected cotton lines derived from gamma irradiated varieties [electronic resource].

Includes references.

Twenty selected cotton lines derived from the two irradiated varieties; Dandara and Giza 81 by ganuna rays were electrphoreticaly analyzed in M5 generation, for water soluble, non-soluble proteins and four enzyme systems; EST, MDH, ADH and G-6PDH. The discrimination of such lines based upon the absence or presence of a unique band(s) exhibited outstanding differences in their banding patterns and occurrence of substantial genetic variations between such lines. The genetic variability observed was sufficient to identiJY uniquely all the 20 lines according to their banding patterns. The electrophoretic analyses of proteins revealed some newly induced bands, such as water soluble protein bands with molecular weights of 258, 176, 95, 62, 29 and 3 kDs, and the water non-soluble protein bands with molecular weights of 9 and 28 kDs. Such newly discrete bands, which were not existed in the unirradiated varieties, may be originated as a reflection of gamma radiation effects, which consequently fixed in such lines thereby, each individual line became distinctive in its own characters as a result forward genetic stability over five generations.

Summary in Arabic.

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