The yeast rhodotorula spp. and candida spp. as a recipent eukaryotes in trans-kingdom conjugation [electronic resource].

Includes references.

Conjugation is often very efficient between members of a given species or genus, it can also occur at a lower rate between phylogenetically distant microorganisms with structurally distinct cell surfaces. This study was attempted to carry out trans-kingdom between E. coli as donor and Rhodotorula spp., Candida spp. yeasts as recipient. Constructed plasmids (pJan 25 and pGW533) derived from R4pGWB conjugative plasmid were used. A conjugation mixture consisted of diparental mating between E. coli harboring one plasmid and Rhodotorula or Candida. Plasmid DNA isolation and gel electrophoresis for transconjugants yeast cells indicated the presence of corresponding bands to donor DNA plasmids. The results clearly indicated that, two plasmids were able to transfer to yeast cells. The transfer was started at one h after mating and the frequency was increased after two to 24h., these were established by detection of B- glucuronidase (gus) marker gene located on plasmids using specific PCR.Tbis is a good proof that, gene transfer through conjugation may be occur from prokaryotes to eukaryotes.

Summary in Arabic.

1