Anticoccidial activity of black cumin (Nigella sativa) in rabbits [electronic resource].

Includes references.

A total of 60 males new zealand white rabbits (free from coccidian infection as Determined by coccidian-free fecal samples over 7 consecutive days) were weighed And distributed into five equal groups of 12 rabbits each. First group (g1) was left as Uninfected and untreated (negative control), second group (g2) was left as uninfected And treated with n sativa seed oil. Rabbits of three other groups (g3, g4) and g5) Were inoculated with 1xl04 sporulated oocysts of eimeria stiedae per each rabbit Through a gastric tube. The third group (g3) was left untreated and considered as Positive control. At day 14 post inoculation (pi), the rabbits of fourth group (g4) were Treated with n sativa seed oil in a dose of 500 mg/kg bw for successive 14 days. Meanwhile, the rabbits of fifth group (g5) were given n sativa seed oil for 7 days Before infection and continued for 28 days pi with the same dose. Fecal oocysts were Collected and counted using a modified mcmaster technique on days 0, 7, 14, 21, 28 And 35 pi. At 28 day pi, blood samples were collected and the serum was prepared and Kept in deep freezer at -20 •c for determination of serum ast, alt, total cholesterol, Creatinine, total protein and albumin. On the 28th day pi, bilary oocysts were counted; Liver lesions were scored and the relative liver weights were reported in five rabbits From each group. As well as fresh liver tissue specimens of the sacrificed rabbits were Fixed in 10% formalin. Sections 5-7 um thickness were prepared and stained with Hematoxylin and eosin (h&e) for the histopathological examination.

Summary in Arabic.