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Evaluation of one bioagent and an insect growth regulator against the cotton leafworm using PCR [electronic resource].

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Abdel-Wahed, M. S

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Language: English Summary language: Arabic Description: p.525-538Other title:

تقييم فاعلية مركب حيوي ومنظم نمو حشري ضد دودة ورق القطن باستخدام تقنيه التوصيف الجزيئي [Added title page title]

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Egyptian journal of agricultural research, 2013 v. 91 (2) [electronic resource].

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In: Egyptian Journal of Agricultural Research 2013.v.91(2)Summary: This study was carried out on 6th instar larvae of Spodoptera littoralis treated in 2nd instar larvae with LC₅₀ of Match and Protecto at 0.0057 ppm, and 0.1099 gm/ml., respectively, Five random primers were used in this study to generate a fragmenting pattern as a tool to investigate the molecular differences between treated samples and control. The numbers of unique and common fragments generated by using these primers (OPO1, OPO2, OPO3, OPO4 and OPO5) was recorded. It has been found that primer OPO2 was the most powerful in generating a unique informative fragmenting pattern, it gave 4 specific unique fragments. The primer OPO4 was the poorest one in generating an informative fragmenting pattern.

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This study was carried out on 6th instar larvae of Spodoptera littoralis treated in 2nd instar larvae with LC₅₀ of Match and Protecto at 0.0057 ppm, and 0.1099 gm/ml., respectively, Five random primers were used in this study to generate a fragmenting pattern as a tool to investigate the molecular differences between treated samples and control. The numbers of unique and common fragments generated by using these primers (OPO1, OPO2, OPO3, OPO4 and OPO5) was recorded. It has been found that primer OPO2 was the most powerful in generating a unique informative fragmenting pattern, it gave 4 specific unique fragments. The primer OPO4 was the poorest one in generating an informative fragmenting pattern.

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