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Preparation of mycoplasma gallisepticum subunit vaccine and its evaluation in chickens [electronic resource].

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El-Shater, S. A. A

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Description: p.157-169Uniform titles:

Veterinary medical journal, 2002 v.50 (1) [electronic resource].

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In: Veterinary Medical Journal 2002.v.50(1)Summary: A 64 kilodalton (KD) lipoprotein was isolated from Mycoplasma gallisepticum membrane and used for preparation of subunit vaccine. Evaluation of the prepared vaccine against colonization of M. gallisepticum (MG) in chicken trachea was studied. Immunoblot of 64 KD protein of M. gallisepticum separated by high performance liquid chromatography (HPLC) and Triton X -114 extraction, against anti- MG chicken serum proved that it is highly immunogenic protein. Sixty oneday old mycoplasma free Hubbard chicks were used. The chicks were divided into 5 groups each of tweleve chicks. Three groups were given 0.25, 0.5 and 0.75 mg of MG vaccine then challenged one month later with 15000 CFU/ 0.1 ml /bird/ intratracheally. The remaining two groups were kept as negative and positive controls. Chickens vaccinated with subunit vaccine (0.5 and 0.75 mg/ bird) resulted in 100% protection against tracheal M. gallisepticum colonization.

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A 64 kilodalton (KD) lipoprotein was isolated from Mycoplasma gallisepticum membrane and used for preparation of subunit vaccine. Evaluation of the prepared vaccine against colonization of M. gallisepticum (MG) in chicken trachea was studied. Immunoblot of 64 KD protein of M. gallisepticum separated by high performance liquid chromatography (HPLC) and Triton X -114 extraction, against anti- MG chicken serum proved that it is highly immunogenic protein. Sixty oneday old mycoplasma free Hubbard chicks were used. The chicks were divided into 5 groups each of tweleve chicks. Three groups were given 0.25, 0.5 and 0.75 mg of MG vaccine then challenged one month later with 15000 CFU/ 0.1 ml /bird/ intratracheally. The remaining two groups were kept as negative and positive controls. Chickens vaccinated with subunit vaccine (0.5 and 0.75 mg/ bird) resulted in 100% protection against tracheal M. gallisepticum colonization.

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