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Genetically modified potato (Solanum tuberosum L.) resistant to potato tuber moth (Phthorimaea operculella) [electronic resource

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Ibrahim, M.A

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Language: English Summary language: Arabic Description: p.1-10Other title:

نباتات بطاطس معدلة وراثيا لمقاومة فراشة درنات البطاطس [Added title page title]

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Arab journal of biotechnology, 2002 v. 5 (1) [electronic resource]:

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In: Arab Journal of Biotechnology 2002.v.5(1)Summary: Potato tuber moth (PTM), Phthorimaea operculella, causes tremendous economic losses to potato production in Egypt. An Egyptian isolate of the entomopathogenic bacteria Bacillus thuringiensis (Bt), isolate CI2, produces a potent Cry1Aa7 toxin that kills the larval stages of PTM, more efficiently than other standard Bt toxins. The cry1Aa7 gene cloned in the binary vector pBI121 and pBI-cry7 construct was transformed to Agrobacterium tumefaciens strain LBA4404 viaBtriparental mating. Transformed LBA4404 strains harboring pB1-cry7 were used to mobilize the cry1Aa7 cassette into the potato cultivar Desiree. Out of 91 transformants, five individual lines (D8, D9, D13, D26 and D37) were shown to be transgenic for cry1Aa7. The integrated toxin gene as well as the expressed Cry1Aa7 was confirmed using polymerase chain reaction (PCR) and enzyme-linked immunosorbant assay (ELISA), respectively.

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Potato tuber moth (PTM), Phthorimaea operculella, causes tremendous economic losses to potato production in Egypt. An Egyptian isolate of the entomopathogenic bacteria Bacillus thuringiensis (Bt), isolate CI2, produces a potent Cry1Aa7 toxin that kills the larval stages of PTM, more efficiently than other standard Bt toxins. The cry1Aa7 gene cloned in the binary vector pBI121 and pBI-cry7 construct was transformed to Agrobacterium tumefaciens strain LBA4404 viaBtriparental mating. Transformed LBA4404 strains harboring pB1-cry7 were used to mobilize the cry1Aa7 cassette into the potato cultivar Desiree. Out of 91 transformants, five individual lines (D8, D9, D13, D26 and D37) were shown to be transgenic for cry1Aa7. The integrated toxin gene as well as the expressed Cry1Aa7 was confirmed using polymerase chain reaction (PCR) and enzyme-linked immunosorbant assay (ELISA), respectively.

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