Regeneration via somatic embryogenesis and microprojectile mediated co-transformation of sugarcane [electronic resource].

Includes references.

Regeneration of sugarcane (Saccharum officinarum) Egyptian cultivar GT54-C9' was performed via somatic embryogenesis through tissue culture. Embryogenic calli differentiated when 2-4 mm segments the leaf base segments were used as explants. Different concentrations of 2,4-D (1.0, 2.0, 3.0 and 4.0 mg/l) were evaluated to optimize the production of embryonic calli. Derived calli were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mgll 2,4-D, 10% coconut water, 500 mg/l casein hydrolysate, 2.8 g/l phytagel and 30 g/l sucrose. Embryos were allowed to develop into plantlets within 20 days from transferring the embryogenic calli to maturation medium (MS medium supplemented with 5% coconut water). A two week old callus initiation culture (C9) was bombarded with tungsten particles coated with pBIl2l plasmid DNA containing the B-glucuronidase GUS reporter gene and neomycin phosphotransferase (NPT-II) selectable marker. Stable transformation was obtained following microprojectile bombardment with the plasmid under control of the 35S promoter.

Summary in Arabic.

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