Thiamine and Pyridoxine alleviate oxidative damage by copper stress in green alga Chlorella vulgaris [electronic resource].

Includes references.

Thiamine and pyridoxine were investigated for their capacity to alleviate oxidative damage by copper stress on a local alga (C. vulgaris). Lower levels ofCu+2 induced a slight stimulation in growth criteria (growth rate & dry weight), photosynthetic pigments (0.5UM) and Orevolution (0.5, 10 !J.M) of C. vulgaris which were inhibited by high Cu +2 concentrations. In contrast, 02-uptake was retarded at the lower Cu +2 levels then significantly increased by increasing the Cu +2 in algal cultures. Proline, MDA contents, and antioxidant enzyme activity of C. vulgaris markedly increased under Cu+2 stress. On the other hand, addition of thiamine or pyridoxine alleviated the oxidative damage of Cu +2 on C. vulgaris growth and enhanced growth, pigment contents, 02- evolution, and antioxidant enzyme activity in the algal cultures compared to reference controls. While O2-uptake, proline content, and lipid peroxidation levels were decreased, thiamine or pyridoxine scavenger systems might be important for supporting the ability of C. vulgaris to resist copper toxicity. Keywords: Copper sulfate, Green alga, Thiamine, Pyridoxine, Antioxidant enzyme, Lipid peroxidation, Proline.

Summary in Arabic.

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