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Low density lipoproteins as cryoprotectants of rabbit semen cryopreservation [electronic resource].

By:

El-Siefy, E. M. E

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Language: English Summary language: Arabic Description: p.43-56Other title:

الليبوبروتنيات منخفضة الكثافة كواقيات للتجميد لحفظ السائل المنوي للأرانب [Added title page title]

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kafrelsheikh veterinary medical journal, 2014 v. 12 (1) [electronic resource].

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In: Kafrelsheikh Veterinary Medical Journal 2014.v.12(1)Summary: The present study was carried out at the International livestock management training center, Sakha, K.frelsheikh, belonging to Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Egypt; during the period from March to May 2013. Aim of this study was to evaluate sperm characteristics and fertility of rabbit buck semen cryopreserved in tris-extender containing different levels of low density lipoproteins (LDL) as replacement of egg yolk. Semen was collected twice weekly form twenty APRI rabbit bucks using artificial vagina. Only ejaculates with≥ 70% mass motility were used for dilution and processing. Four extenders were used: Tris 20% egg yolk extender as a control, and substitution of whole egg yolk with 8, 10 and 12% LDL. Semen was diluted and packaged into 0.25 ml straws, cooled, held at 5°C for 4 h, and then frozen in liquid nitrogen and stored at -196°C. Percentages of progressive motility, livability, abnormality and intact acrosome were determined at post- dilution,-equilibration, -thawing.

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The present study was carried out at the International livestock management training center, Sakha, K.frelsheikh, belonging to Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Egypt; during the period from March to May 2013. Aim of this study was to evaluate sperm characteristics and fertility of rabbit buck semen cryopreserved in tris-extender containing different levels of low density lipoproteins (LDL) as replacement of egg yolk. Semen was collected twice weekly form twenty APRI rabbit bucks using artificial vagina. Only ejaculates with≥ 70% mass motility were used for dilution and processing. Four extenders were used: Tris 20% egg yolk extender as a control, and substitution of whole egg yolk with 8, 10 and 12% LDL. Semen was diluted and packaged into 0.25 ml straws, cooled, held at 5°C for 4 h, and then frozen in liquid nitrogen and stored at -196°C. Percentages of progressive motility, livability, abnormality and intact acrosome were determined at post- dilution,-equilibration, -thawing.

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