Isolation and sequencing of Myf5 gene (promoter region) from Beheiry Egyptian water buffaloes [electronic resource].

Includes references.

Myogenic factor 5 (Myf5) is a member in the myogenic regulatory factor (MRF) family of basic helix-loop-helix transcription factors, which is involved in the differentiation and maturation of myotubes and is highly expressed postnatally. The sequences of this gene are well known in cattle and small ruminants but in buffalo no available sequences were previously recorded. Therefore, the current investigation threw the light on the isolation and sequencing the promoter region of this gene, which is the most appropriate portion of Myf5 that contains single nucleotide polymorphisms (SNPs), in Egyptian buffalo. To achieve this task, PCR was performed in which gene-specific primers flanking to promoter region of Myf5 were utilized. A PCR products of 700bp, of Myf5 gene corresponding to the expected product size, was successfully amplified. Sequencing of the purified PCR products.

Summary in Arabic.

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