Yield, quality, and molecular markers of new lentil mutant lines [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: p.423-440Other title:
  • المحصول وصفات الجودة والمعلمات الجزيئية لسلالات جديدة مطفرة من العدس [Added title page title]
Uniform titles:
  • Egyptian journal of agricultural research, 2015 v. 93 (2) B, Special issue [electronic resource]:
Subject(s): Online resources: In: Egyptian Journal of Agricultural Research 2015.v.93(2)B,SISummary: The present study aimed to characterize 17 new lentil mutant lines for their seed yield, daylj. to maturity, physical, . chemical, and molecular characteristics in comparison with their mother lentil cultivar 'Giza 9' and two common cultivars 'Sinai 1' and 'Giza 51'. In addition, genetic variation and relatedness of the highest yielding eight lentil lines along' with Giza 9, out of the 17 tested lines were investigated by PCR based RAPD technique. The field trials were. conducted at Gemmeiza Research Station, ARC, Egypt, during 201112012 and 2012/2013 winter seasons, and the laboratory investigations were made in the labofatory of Seed Technology Research Department, ARC during 2013/2014. The average seed yi,eld of the 17 mutant lines wen~ 4.64 ardab fed:1 , indicating their , superiority over the three check cultivars. Moreover, eight mutant lines produced higher seed yield ranging from 4.85 to 5.99 ardab fed:1 , and exceeded their mother cultivar Giza 9 by 29-59.3%. These eight lines also exceeded Giza 9 in their ·seed phosphorus and potassium content. Siriai 1 cultivar was the earliest in maturity, where it matured at 119 days. Giza 9 was the best in electrical conductivitY test, while the hydratio11 coefficients before (HCB) and after cooking (HCA) indicated that the 17 mutant lines have better seed quality than Giza 9. Molecular analysis of nine lentil lines showed that a total of 94 markers were amplified of which 35.1 % were polymorphic. Genetic similarity among the genotypes revealed a strong relationship between mutant lines M48 and M49 (similarity inde)< 100%), while a weak relationship was observed between line M40 and M46 (similarity index 87%). · Cluster analysis divided the nine lines of lentil into three main cl&sters. All genotypes except lines M48 and M49 could be discriminated from one another using RAPD-PCR. Five bands were found to be u5eful as genotype-specific markers. Despite the observed low level of genetic diversity, genetic differences existed among Giza 9 and other eight mutant lines. Keywords: Lens culinaris L., Mutant lentils, 'similarity index, RAPD technique.
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The present study aimed to characterize 17 new lentil mutant lines for their seed yield, daylj. to maturity, physical, . chemical, and molecular characteristics in comparison with their mother lentil cultivar 'Giza 9' and two common cultivars 'Sinai 1' and 'Giza 51'. In addition, genetic variation and relatedness of the highest yielding eight lentil lines along' with Giza 9, out of the 17 tested lines were investigated by PCR based RAPD technique. The field trials were. conducted at Gemmeiza Research Station, ARC, Egypt, during 201112012 and 2012/2013 winter seasons, and the laboratory investigations were made in the labofatory of Seed Technology Research Department, ARC during 2013/2014. The average seed yi,eld of the 17 mutant lines wen~ 4.64 ardab fed:1 , indicating their , superiority over the three check cultivars. Moreover, eight mutant lines produced higher seed yield ranging from 4.85 to 5.99 ardab fed:1 , and exceeded their mother cultivar Giza 9 by 29-59.3%. These eight lines also exceeded Giza 9 in their ·seed phosphorus and potassium content. Siriai 1 cultivar was the earliest in maturity, where it matured at 119 days. Giza 9 was the best in electrical conductivitY test, while the hydratio11 coefficients before (HCB) and after cooking (HCA) indicated that the 17 mutant lines have better seed quality than Giza 9. Molecular analysis of nine lentil lines showed that a total of 94 markers were amplified of which 35.1 % were polymorphic. Genetic similarity among the genotypes revealed a strong relationship between mutant lines M48 and M49 (similarity inde)< 100%), while a weak relationship was observed between line M40 and M46 (similarity index 87%). · Cluster analysis divided the nine lines of lentil into three main cl&sters. All genotypes except lines M48 and M49 could be discriminated from one another using RAPD-PCR. Five bands were found to be u5eful as genotype-specific markers. Despite the observed low level of genetic diversity, genetic differences existed among Giza 9 and other eight mutant lines. Keywords: Lens culinaris L., Mutant lentils, 'similarity index, RAPD technique.

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