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Biochemical markers associated with soil-born and foliar disease resistance of high yielding canola mutants [electronic resource].

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Azzam, Clara R

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Language: English Summary language: Arabic Description: p.467-496Other title:

المعلمات البيوكيميائية المرتبطة بمقاومة أمراض التربة والمجموع الخضري لطفرات الكانولا ذات الإنتاجية العالية [Added title page title]

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Egyptian journal of agricultural research, 2015 v. 93 (2) B, Special issue [electronic resource]:

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In: Egyptian Journal of Agricultural Research 2015.v.93(2)B,SISummary: Nineteen canola (Brassica napus L.) mutants developed by gamma ray in previous generations and their parental cultivars: Bactol, Linetta and Conny, were investigated for their resistance to charcoal rot, fusarium wilt, alternaria leaf spot and powdery mildew diseases under greenhouse and field conditions during the winter seasons 2012-2013 and 2013-2014. Mutants CMl, CM2, CMS, CM12, CM14, CM17 and CM19 were the most resistant ones, while the three parental cultivars, CM9, CM16 and CM18 were the most susceptible ones. Based on protein profile of the 19 mutants and their parental cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SOS-PAGE) technique, SOS-PAGE profile showed some polypeptides associated with resistance and susceptibility to the diseases under investigation. Wide range of similarity index was observed among each parental cultivars and its developed mutants. The UPGMA based dendrogram showed that all the most resistant mutants to charcoal rot, fusarium wilt, alternaria leaf spot and powdery mildew diseases were gathered together as did the most susceptible ones. Key Words: Brassica napus L., mutants, yield and yield components, charcoal rot, fusarium wilt, alternaria leaf spot, powdery mildew, SOS-PAGE, Biochemical markers and cluster analysis.

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Nineteen canola (Brassica napus L.) mutants developed by gamma ray in previous generations and their parental cultivars: Bactol, Linetta and Conny, were investigated for their resistance to charcoal rot, fusarium wilt, alternaria leaf spot and powdery mildew diseases under greenhouse and field conditions during the winter seasons 2012-2013 and 2013-2014. Mutants CMl, CM2, CMS, CM12, CM14, CM17 and CM19 were the most resistant ones, while the three parental cultivars, CM9, CM16 and CM18 were the most susceptible ones. Based on protein profile of the 19 mutants and their parental cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SOS-PAGE) technique, SOS-PAGE profile showed some polypeptides associated with resistance and susceptibility to the diseases under investigation. Wide range of similarity index was observed among each parental cultivars and its developed mutants. The UPGMA based dendrogram showed that all the most resistant mutants to charcoal rot, fusarium wilt, alternaria leaf spot and powdery mildew diseases were gathered together as did the most susceptible ones. Key Words: Brassica napus L., mutants, yield and yield components, charcoal rot, fusarium wilt, alternaria leaf spot, powdery mildew, SOS-PAGE, Biochemical markers and cluster analysis.

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