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Identification and enzyme characterization of some E.coli serogroups causing enteritis in buffaloes [electronic resource].

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Helal, A. D

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Language: engbara Description: p.121-131Other title:

استخدام الطرق التقليدية والسيتوكيميائية الكمية للتعرف على بعض العترات السيرولوجية للميكروب القولونى المسببة للالتهابات المعوية فى الجاموس والتوصيف الإنزيمى لها [Added title page title]

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Assiut veterinary medical journal, 2014 v. 60 (142) [electronic resource].

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In: Assiut Veterinary Medical Journal 2014.v.60(142)Summary: Enteritis ochemical enzyme characterization of the different identified serogroups were carried out through the cytochemical quantitative determination of bacterial mitochondrial Adenosene-tri phosphatase (ATP-ase) and succinic dehydrogenase (SDH-ase) enzyme activities for their possible variations among the isolated and identified E.coli serogroups. Four values to each of E.coli serogroup (2 for each enzyme) as optical densities (OD.) could be obtained: (ATP- ase activity & Actual ATP-ase reaction and SDH-ase activity & Actual SDH-ase reaction) for E.coli-serogroups: E.coli 026, E.coli 055, E.coli 078, E.coli 086 and E.coli 0111 as following: (0.595 & 0.285 and 0.560 & 0.210), (0.565 & 0.265 and 0.550 & 0.200), (0.590 & 0.255 and 0.540 & 0.190), (0.590 & 0.205 and 0.490 & 0.180) and (0.600 & 0.160 and 0.560 & 0.150) nm respectively.

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Enteritis ochemical enzyme characterization of the different identified serogroups were carried out through the cytochemical quantitative determination of bacterial mitochondrial Adenosene-tri phosphatase (ATP-ase) and succinic dehydrogenase (SDH-ase) enzyme activities for their possible variations among the isolated and identified E.coli serogroups. Four values to each of E.coli serogroup (2 for each enzyme) as optical densities (OD.) could be obtained: (ATP- ase activity & Actual ATP-ase reaction and SDH-ase activity & Actual SDH-ase reaction) for E.coli-serogroups: E.coli 026, E.coli 055, E.coli 078, E.coli 086 and E.coli 0111 as following: (0.595 & 0.285 and 0.560 & 0.210), (0.565 & 0.265 and 0.550 & 0.200), (0.590 & 0.255 and 0.540 & 0.190), (0.590 & 0.205 and 0.490 & 0.180) and (0.600 & 0.160 and 0.560 & 0.150) nm respectively.

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