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Nutritional and enviromental factors affecting Cellulases activity produced by high potent Cellulolytic Bacilli [electronic resource].

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Abou -Taleb, Khadiga A

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Language: English Summary language: Arabic Description: p.29-39Other title:

العوامل الغذائية والبيئة المؤثرة على نشاط السليوليز المنتج بواسطة سلالتين من العصويات ذات الكفاءة العالية المحللة للسليلوز [Added title page title]

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Mansoura University journal of agricultural chemistry and biotechnology, 2015 v.6 (2) [electronic resource].

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In: Mansoura University Journal of Agricultural chemistry and biotechnology 2015.v.6(2)Summary: Effect of some nutritional and environmental factors on growth and cellulase production by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 was investigated. Results indicated that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and nitrogen sources respectively. Initial pH 7 and 3% inoculum size (3.5-4.3 x106 CFU/ml) found to be optimal for growth and ceUulase production. Incubation temperature cat 30°C and 45°C achieved the highest activity of cellulase for Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 respectively, and the suitable shaking rate was 150 and 200 rpm.

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Effect of some nutritional and environmental factors on growth and cellulase production by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 was investigated. Results indicated that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and nitrogen sources respectively. Initial pH 7 and 3% inoculum size (3.5-4.3 x106 CFU/ml) found to be optimal for growth and ceUulase production. Incubation temperature cat 30°C and 45°C achieved the highest activity of cellulase for Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 respectively, and the suitable shaking rate was 150 and 200 rpm.

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