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Effect of different hormonal treatments as therapeutic procedure on repeat breeder buffalo heifers [electronic resource].

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Abdel-Khalek, A. E

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Language: English Summary language: Arabic Description: p.257-266Other title:

تأثير المعاملات الهرمونية كإجراء علاجي على عجلات الجاموس متكررة التلقيح [Added title page title]

Uniform titles:

Egyptian journal of animal production, 2012 v. 49 (3) [electronic resource].

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In: Egyptian journal of animal production 2012.v.49(3)Summary: Total of 30 Egyptian buffalo heifers (20-25 months and 340-360 kg) failed to conceive after 3 services (repeat breeder heifers) were exposed to three hormonal protocols after exhibiting the 41" estrous cycle. In the 1st protocol, heifers (n= 10) were injected with GnRH on day of estrus and service. In the 2nd protocol, heifers (n=10) were injected with GnRH on day of estrus (0 day), followed by PGF2a on day 7. Heifers displaying estrus within 48 hr of PGF;a. injection were sen,ed, while those did not show estrus within 48 h were injected with a second dose of GnRH on day 9 and served on day 10. In the 3rd protocol, he(fers (n=10) were injected with F1 PGF2a injection starting on day 6 after exhibiting the 4rl' estrous cycle. Animals exhibiting estrus within 72 hr after F1 PGF2a injection were sen,ed, while those did not exhibit estrus were re-injected after 11 days of the F1 injection with 2"d PGF2a and served 24-48 hr later. Pregnancy was diagnosed by ultrascan after 35.d of service. At sen1ice, diameter of the largest follicles was recorded for the F1 and 2"d protocols, while diameter of corpus luteum (CL) was recorded for the 3rd protocol using ultrascan. Blood samples were collected pre- and post- hormonal injections of each protocol for determination of progesterone (P4) concentrations in blood serum of all animals. Results showed that conception rate (CR) in the F1 protocol was 80% and mean diameter of the largestfollicle was 13.25 and 9.10 mm in conceived and non-conceived heifers, respectively.

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Total of 30 Egyptian buffalo heifers (20-25 months and 340-360 kg) failed to conceive after 3 services (repeat breeder heifers) were exposed to three hormonal protocols after exhibiting the 41" estrous cycle. In the 1st protocol, heifers (n= 10) were injected with GnRH on day of estrus and service. In the 2nd protocol, heifers (n=10) were injected with GnRH on day of estrus (0 day), followed by PGF2a on day 7. Heifers displaying estrus within 48 hr of PGF;a. injection were sen,ed, while those did not show estrus within 48 h were injected with a second dose of GnRH on day 9 and served on day 10. In the 3rd protocol, he(fers (n=10) were injected with F1 PGF2a injection starting on day 6 after exhibiting the 4rl' estrous cycle. Animals exhibiting estrus within 72 hr after F1 PGF2a injection were sen,ed, while those did not exhibit estrus were re-injected after 11 days of the F1 injection with 2"d PGF2a and served 24-48 hr later. Pregnancy was diagnosed by ultrascan after 35.d of service. At sen1ice, diameter of the largest follicles was recorded for the F1 and 2"d protocols, while diameter of corpus luteum (CL) was recorded for the 3rd protocol using ultrascan. Blood samples were collected pre- and post- hormonal injections of each protocol for determination of progesterone (P4) concentrations in blood serum of all animals. Results showed that conception rate (CR) in the F1 protocol was 80% and mean diameter of the largestfollicle was 13.25 and 9.10 mm in conceived and non-conceived heifers, respectively.

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