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Assessment of genetic variability and genotyping of some Citrus accessions using molecular markers [electronic resource].

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Hussein, Ebtissam H. A

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Language: English Summary language: Arabic Description: P. 23-36Other title:

دراسة التباين الوراثي و تحديد البصمة الوراثى لبعض أصناف الموالح باستخدام الواسمات الجزيئية [Added title page title]

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Arab journal of biotechnology, 2004 v. 7 (1) [electronic resource]:

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In: Arab Journal of Biotechnology 2004.v.7(1)Summary: Genotyping of 14 Citrus accessions was carried out using RAPD and SCAR markers. The genetic variability among the 14 Citrus accessions was estimated using forty decamer RAPD primers. The total number of amplicons detected was 531, including 349 polymorphic amplicons. This represents a level of polymorphism of 65.7% and an average number of 8.7 polymorphic bands per primer. RAPD markers detected genetic similarity ranging from 10.8% to 87.7% between TC and each of WM, A WM and BSO and between MSG and RRG, respectively. The similarity matrices were employed in the cluster analysis to generate a dendrogram using the UPGMA method. The dendrogram separated Troyer citrange from the other Citrus genotypes. Moreover, accessions belonging to the same species always clustered together.

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Genotyping of 14 Citrus accessions was carried out using RAPD and SCAR markers. The genetic variability among the 14 Citrus accessions was estimated using forty decamer RAPD primers. The total number of amplicons detected was 531, including 349 polymorphic amplicons. This represents a level of polymorphism of 65.7% and an average number of 8.7 polymorphic bands per primer. RAPD markers detected genetic similarity ranging from 10.8% to 87.7% between TC and each of WM, A WM and BSO and between MSG and RRG, respectively. The similarity matrices were employed in the cluster analysis to generate a dendrogram using the UPGMA method. The dendrogram separated Troyer citrange from the other Citrus genotypes. Moreover, accessions belonging to the same species always clustered together.

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