Development of AFLP markers and genotyping of elite maize inbred lines [electronic resource].

Includes references.

The AFLP technology was used to develop AFLP markers characterizing 10 maize inbred lines and generating unique fingerprint for each inbred line. Selective amplification of the genomic DNA from the ten inbred lines, using eighteen primer combinations, revealed a total number of 1143 amplicons. The number of polymorphic amplicons across the 10 inbred lines was 902, representing a level of polymorphism of 78.9%. Genetic distances between lines were calculated from AFLP data using the Dice coefficient. UPGMA analysis and dendrogram clustered the ten inbred lines into two main clusters each composed of different groups revealing relationships which were basically consistent with the pedigree of the inbred lines. Moreover, unique AFLP markers characterizing each of the ten inbred lines were identified. The total number of unique markers was 122 positive markers and 73 negative markers.

Summary in Arabic.

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