Molecular characterization of toxigenic and antibiotic resistant Of Staphylococcus Aureus of recurrent bovine mastitis [electronic resource]
Language: English Summary language: Arabic Description: p.1-8Other title:- الصفات الجزيئية لسموم المكور العنقودى الذهبى المقاوم لبعض المضادات الحيوية لالتهاب الضرع المتكرر فى الابقار الحلابة [Added title page title]
- Assiut veterinary medical journal, 2018 v. 64 (158) [electronic resource].
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Main | ART AVMJ V64 NO158 1 (Browse shelf(Opens below)) | Available |
Includes bibliographic reference
To enhance the diagnosis of Staphylococcus aureus mastitis and its prospective antibiotic resistance in dairy
cattle, a multiplex polymerase chain reaction (PCR) assay was developed for concurrent species identifycation,
detection antibiotic resistant phenotypically and genotypically to penicillin (blaZ gene), gentamycin (aac(6') aph
(2'')genes), and tetracycline (tetK, gene) and recognize the toxogenic patterns of S. aureus in cow’s milk with
recurrent clinical mastitis. Bacterial culturing was carried out on 179 bovine recurrent clinical mastitic milk
samples resulted in 84 (46.9%) coagulase positive staph isolates, while 69 (38.5%) confirmed by molecular
identification by PCR as S. aureus. Also some virulence factors as enterotoxin A and B (Sea and Seb) genes
were determined in 7(10.1%) and 1 (1.4%) respectively in confirmed isolates. As well as determined drugs
resistance and their genes. The results of antimicrobial susceptibility tests showed 100%. 43.5% and 58%
resistance for penicillin, gentamycin and tetracycline respectively. While genotypically were 100%, 40.6% and
53.6% for (blaZ, aac (6') aph (2'') and tetK, respectively. This study reports the presence of multidrug resistant
S. aureus in recurrent clinical mastitis with highly virulent toxic genes that could be a major obstacle in the
treatment and control of mastitis in dairy farms causing highly economic impacts and recommended that the
polymerase chain reaction (PCR)-based assays to detect pathogens associated with mastitis and that it has several
advantages, including rapid results and high sensitivity.
Summary in Arabic
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