Molecular characterization of two selected pigeon Paramyxovirus-1 isolates reveals two different cleavage site amino acid motifs [electronic reource].
Language: English Summary language: Arabic Description: p. 1-10Uniform titles:- Alexandria journal of veterinary science, 2018 v. 59 (1) [electronic reource].
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Articles
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Main | ART AJVS V59 NO1 1 (Browse shelf(Opens below)) | Available |
Includes references.
The aim of this study was the isolation, identification and molecular characterization of Avian (Pigeon) Paramyxovirus-1 (APMV-1) isolated
from clinically affected pigeons suspected to be infected with PPMV-1 in Egypt between 2016 and 2017. Twenty-five field samples were collected
and inoculated into the allantoic cavity of ECE. Allantoic fluids were tested for haemagglutinating (HA) activity followed by haemagglutination
inhibition (HI) test for virus identification. Molecular confirmation was done by reverse transcription polymerase chain reaction (RT-PCR) using
primers specific to the fusion (F) gene. Results revealed 12 out of 25 positive samples. Two samples were selected for sequencing and phylogenetic
analysis which revealed that two different amino acid motifs were found at the cleavage site of F protein: 112 KRQKRF117 (associated with virulent strains)
and 112GRQGRL117 (associated with lentogenic strains).To our knowledge, this is the first reported PPMV-1 isolates that possess the sequences of 112GRQGRL117
within the F0 protein.
Summary in Arabic
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