Characterization of aerobic spore-forming bacteria isolated from raw milk, skim milk powder and UHT milk [electronic resource]

By: Contributor(s): Language: English Summary language: Arabic Description: P. 99-111Other title:
  • صفات البكتريا المتجرثمة الهوائية المعزولة من اللبن الخام، اللبن الفرز المجفف و اللبن المعقم [Added title page title]
Uniform titles:
  • Alexandria science exchange journal, 2017 v. 38 (1) [electronic resource]:
Subject(s): Online resources: In: Alexandria Science Exchange Journal 2017.v.38(1)Summary: ABSTRACT Thermoduric and thermophilic aerobic spore-forming bacteria are the common causes of spoilage of pasteurized and Ultra-high-temperature (UHT) milk. The presence of high level of aerobic spore-forming bacteria in raw and recombined milk used in making UHT milk increased the spoilage and/or the reduced shelf life in the final product. In this study, 140 samples of (32) raw milk (RM), (8) skim milk powder (SMP) and (100) UHT milk were collected to isolate aerobic spore-forming bacteria. A total of 210 isolates of heat resistant bacteria were classified according to morphological, physiological and biochemical tests. Out of theses isolates, 144 strains from RM (102), SMP (25) and UHT milk (17) were expected to be Bacillus spp. The isolates were evaluated for proteolytic activity and lactose fermentation, 96.55% of the isolates were able to hydrolyze casein, while 42.76 % of the isolates were able to ferment lactose. Thermophilic Bacillus species were the predominant isolates from raw milk and skimmed milk powder. While, mesophilic Bacillus species were the predominant in UHT milk.
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Articles Articles Main ART ASEJ V38 No1 12 (Browse shelf(Opens below)) Available

Includes references.

ABSTRACT Thermoduric and thermophilic aerobic spore-forming bacteria are the common causes of spoilage of pasteurized and Ultra-high-temperature (UHT) milk. The presence of high level of aerobic spore-forming bacteria in raw and recombined milk used in making UHT milk increased the spoilage and/or the reduced shelf life in the final product. In this study, 140 samples of (32) raw milk (RM), (8) skim milk powder (SMP) and (100) UHT milk were collected to isolate aerobic spore-forming bacteria. A total of 210 isolates of heat resistant bacteria were classified according to morphological, physiological and biochemical tests. Out of theses isolates, 144 strains from RM (102), SMP (25) and UHT milk (17) were expected to be Bacillus spp. The isolates were evaluated for proteolytic activity and lactose fermentation, 96.55% of the isolates were able to hydrolyze casein, while 42.76 % of the isolates were able to ferment lactose. Thermophilic Bacillus species were the predominant isolates from raw milk and skimmed milk powder. While, mesophilic Bacillus species were the predominant in UHT milk.

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