Karyotyping and molecular sexing in Brycinus Nurse (Characiformes, Alestidae) from Nile river in Egypt [electronic resource].
Language: English Summary language: Arabic Description: p.127-131Other title:- التوصيف الكروموسومي والفروق الجزيئيه بين الجنسية فى سمك الراي من نهر النيل في مصر [Added title page title]
- Mansoura University journal of agricultural chemistry and biotechnology, 2019 v. 10 (7) [electronic resource].
| Item type | Current library | Call number | Status | Date due | Barcode | |
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Main | ART MUJACB V10 NO7 1 (Browse shelf(Opens below)) | Available |
Includes bibliographic reference
The present study aimed to investigate the karyotype and molecular sex differences in Brycinus nurse.
Analysis of 430 metaphases spreads from 12 specimens revealed that the diploid chromosome number is
44 chromosomes and the proposed karyotype formula is n = 3 M + 3 SM + 16 A, FN = 56. No morphologically
differentiated heteromorphic sex chromosomes were observed. On the other hand, 29 primers of three
molecular markers (RAPD, SCoT and ISSR) were tested to differentiate between males and females of
B. nurse. From all tested primers only two primers (OPI-18 and SCoT-18) successfully generated
female specific bands in the bulked DNA samples which further confirmed using individual DNA samples.
According to molecular analysis result in this study it seems that females of B. nurse are the
heterogametic gender (ZW) and males of B. nurse are the homogametic gender (ZZ). In the future,
SCoT marker can be used as suitable and powerful marker in genetic analysis studies in fish.
127 - 131
Summary in Arabic
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