Detection and identification of a new isolate of Grapevine fanleaf Virus naturally infecting Grapevine plants in Egypt using qReal Time-PCR [electronic resource]

Includes bibliographic reference

Grapevine fanleaf virus (GFLV) is a member of the genus Nepovirus in the family Comoviridae, a widely
distributed virus responsible for grapevine (Vitis vinifera) degeneration. This virus causes serious economic losses
by reducing grape crop yield. The Quantitative Real-Time Reverse Transcription-Polymerase Chain Reaction
(qReal Time-PCR) assay was carried out on (GFLV) recovered from infected grapevines leaves at Alexandria,
Egypt. A 606 bp fragment of the GFLV RNA-2 coat protein (CP) gene was amplified and then sequenced. Results
of reactions of diagnostic hosts were observed on Gomphrena globosa, which developed systemic mottling,
leaves twisting and necrotic spots during spring, whereas Chenopodium amaranticolor induced systemic mottling
and leaf deformation, and its sap seemed relatively insensitive to the inhibitors of infection. Mottling of Glycine
max was detected after inoculation, but inoculation of Nicotiana glutinosa didn't induce any symptoms. This
study aimed to detect and identify a new isolate of GFLV-DA3 from Egypt using biological and molecular tools.

Summary in Arabic