Prevalence and multidrug resistance profiles of several bacterial pathogens isolated from hospital inanimate surfaces in Faisalabad, Pakistan [electronic resource]

Includes bibliographic reference

Hospital environment and inanimate surfaces are considered as potential sources of opportunistic and
nosocomial pathogens. Indirect transmission of microbes from the hospital surfaces has a major role in hospital
acquired nosocomial infections and their colonization. The present study was designed to investigate the
prevalence of major pathogenic bacteria isolated from hospital inanimate surfaces. Random swab samples were
taken from inanimate surfaces and apparatus used in daily treatment of patients from the major hospitals in district
of Faisalabad, Pakistan. These swab samples were cultivated on suitable culture media including; nutrient agar,
MacConkey’s agar and blood agar to isolate the bacterial pathogens. Identification of the bacterial cultures was
carried out by observing the cultural and macroscopic characteristics including Gram staining. Further verification
of these bacterial cultures was carried out using appropriate biochemical assays. The biochemical assays were
performed for characterization of Staphylococcus aureus, Staph. epidermidis, Staph. saprophyticus, Pseudomonas
aeruginosa, Klebsiella pneumonia and Escherichia coli. These pure cultures were then tested for resistance to
different antibiotics using the Kirby–Bauer method. The prevalence of Gram positive bacteria (60.83%) was more
than that of Gram negative bacteria (24.13%). The results of antibiotics susceptibility tests were analyzed
statistically.

Summary in Arabic