Extraction and purification of lovastatin from the edible mushroom Laetiporus sulphureus and its antioxidant activity [electronic resource]
Language: English Summary language: Arabic Description: 169 - 175 pOther title:- استخلاص وتنقي Lovastatin من الفطر Laetiporus sulphureus وتحديد فعاليته المضادة للاكسدة [Added title page title]
- Egyptian journal of botany, 2022 v. 62 (1) [electronic resource]
| Item type | Current library | Call number | Status | Date due | Barcode | |
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Articles
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Main | ART EJB V62 No1 14 (Browse shelf(Opens below)) | Available |
Includes bibliographic reference
Lovastatin is a cholesterol-lowering drug produced by the secondary metabolism ofdifferent fungi. In the current study, lovastatin was extracted from the basidiomycetes fungus Laetiporus sulphureus by submerged fermentation using an organic solvent ethyl acetate . Initial detection of lovastatin production in the crude extract was carried out using agar well diffusion method and was found to have inhibitory activity against yeast Candida albicans. Thin-layer chromatography (TLC) was used to detected lovastatin, and a distinct spot with an RF value of 0.55 appeared similar to standard Lovastatin. A single peak was also featured with high-performance liquid chromatography (HPLC) with a retention time of 14.61 minutes to see the purity of Lovastatin. The result of the detection of active groups in lovastatin purified by the infrared spectrum indicated the presence of amide and carboxyl groups. The purified lovastatin showed antioxidant efficacy with the DPPH test, with the inhibitory effect reaching 75% at a concentration of 100μg/mL compared with the inhibitory effect of standard antioxidant, ascorbic acid, which reached 85%.
Keywords: DPPH assay, Edible mushroom, HPLC analysis, Laetiporus sulphureus, Lovastatin, TLC.
Summary in Arabic
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