Down regulation of biofilm and quorum sensing genes in E. coli isolated from broiler chickens pericarditis lesions by the action of some essential oils [electronic resource]

Original Research Article

Includes bibliographic reference

E. coli is one of the most important pathogenic bacteria in poultry industry. It
causes high economic losses, high morbidity and mortality rates due to
collibacillosis. Moreover, it is multidrug resistant bacteria. In the last few years,
scientists directed their efforts to the use of essential oils which .. have
antimicrobial actions by inhibition of some virulence properties such as biofilm
formation and quorum sensing. In this study, out of 150 examined samples, 139
were found to be bacteriologically positive for G-ve bacilli (92. 7%) and 84 E. coli
isolates (60.4%) were recovered from broilers suffering from pericarditis.
Twenty random recovered isolates were selected for antimicrobial
susceptibility test. All isolates were completely resistant to amoxicillin,
gentamicin, lincomycin and florfenicol and 900;6 resistant to trimethoprim-
sulfamethoxazole, kanamycin, ciprofloxacin and doxycycline while sensitivity to
amoxicillin+clavulanic acid and cefotaxime was 40% and 20% respectively. We
screened 15 randomly selected MOR isolates by CR assay for detection of
biofilm formation at which CR positive isolates represent 80%. Cinnamon and
clove oils showed antimicrobial effect and cause down regulation in both
biofilm gene (sfa) and quorum sensing gene (luxS).
Keywords Antimicrobial resistance; Biofilm; E. coli; Essential oils; Quorum
sensing; Virulence.

Summary in Arabic