Isolation of lumpy skin disease virus isolated from sppv vaccinated cattle [electronic resource]

Original Research Article

Includes bibliographic reference

Massive outbreaks of suspected LSDV in cattle population were observed
during 2017 and 2018 at Beni-Suef governorate. Samples from diseased
cattle, previously SPPV vaccinated with SPPV, were inoculated in
embryonated chicken eggs (ECE) using chorio-allantoic membrane (CAM)
route. Histopathological examination of the inoculated CAMs showedJarge
eosinophilic intracytoplasmic inclusion bodies characteristic for LSDV. The
inoculated CAMs were hemorrhagic with congestion blood vessels appeared
by the 1st passage then become more pronounced after the second - fourth
passages. Characteristic pock lesions were observed after the 1st passage and
become clear after the third passage and become clearly observed 4 days post
inoculation. For molecular identification, DNA was extracted from a pool of
the infected CAMs. Two pairs of primers specific for LSDV including one
flanking a 554 bp product of the G gene and second flanking 172 of the RP030
gene were used. In conclusion, LSOV infections have been detected and the
virus has been isolated and identified by PCR from cattle previously
vaccinated with SPPV vaccine during the summer of 2018.
Keywords:Egypt; LSDV; PCR; Sheep pox vaccine.

Summary in Arabic