Studies on conservation of gladiolus plants and cormel formation by using tissue culture technique [electronic resource].

Includes references.

The experimental trail was consummated in Plant Tissue Culture Laboratory at El-Zohria Botanical Garden, Horticulture Research Institute, Agriculture Research Center, during 2008 – 2010 years. It intended to find out the most suitable treatments for propagation of Gladiolus primulinus var Atom by using tissue culture technique. Buds from corms of Gladiolus primulinus were effectively surface sterilized with a mixture of Clorox (commercial bleach) and mercuric chloride (HgCl) at 40 % Clorox plus 2.0 g/L Hg22Cl. Explants were transferred to a solidified MS medium containing 1.0 mg /l NAA for establishment stage. The second stage, shoots were transferred to MS medium containing of 2.0 mg/l BA which was used in the current study to improve number of shoots production. Sucrose and MS strength was used to try in vitro producing of higher number of cormels. Sourbitol or manitol and MS medium were more appropriate for germplasm from shoots conservation. The addition of sucrose at 90 g/l to 3/4 MS-strength medium enhanced number of cormels/shoot. T he same and the best results of medium for rooting stage was 3/4 MS + 2 mg/l IBA. Key words: Micropropagation, In vitro, Tissue culture, Gladiolus, Shoot tips, cormel and germplasm.

Summary in Arabic.

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