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Molecular characterization of highly pathogenic avian influenza viruses circulating in commercial broiler chickens in some localities in Sohag province [electronic resource].

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Mohamed, M. A

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Language: English Summary language: Arabic Description: p.1-13Other title:

التوصيف الجزيئى لفيروسات أنفلونزا الطيور المتداولة فى الدجاج اللاحم التجارى من بعض المواقع فى محافظة سوهاج [Added title page title]

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Assiut veterinary medical journal, 2012 v. 58 (133) [electronic resource].

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In: Assiut Veterinary Medical Journal 2012.v.58(133)Summary: chickens experiencing respiratory signs and congestion of head and shank regions and molecularly characterized in Sohag province. The results showed, twenty five (80.6%) of swabs were confirmed positive for carrying type A Avian influenza virus (AIV) by using rapid antigen detection kit. Total RNAs were extracted from the 17 positive AI type A swabs and One step RT-PCR test was performed for subtyping using specific primers targeting H5, H7, N1 and N2. AIV H5 subtype was detected in 9 samples and the rest was untypeable with H7 primers. From the 9 H5 strains, one H5N1 detected and 3 were H5N2 that reflects the presence of two subtypes co-circulated at that time of infection and other untypeable strains with our available primers.

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chickens experiencing respiratory signs and congestion of head and shank regions and molecularly characterized in Sohag province. The results showed, twenty five (80.6%) of swabs were confirmed positive for carrying type A Avian influenza virus (AIV) by using rapid antigen detection kit. Total RNAs were extracted from the 17 positive AI type A swabs and One step RT-PCR test was performed for subtyping using specific primers targeting H5, H7, N1 and N2. AIV H5 subtype was detected in 9 samples and the rest was untypeable with H7 primers. From the 9 H5 strains, one H5N1 detected and 3 were H5N2 that reflects the presence of two subtypes co-circulated at that time of infection and other untypeable strains with our available primers.

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