Evaluation of Mycobacterium Bovis. cell extract antigens for in-vitro diagnosis of Bovine Tuberculosis in experimentally infected guinea pigs [electronic resource].
Language: English Summary language: English Description: p. 261-276Uniform titles:- Veterinary medical journal , 2001 v.49 (2) [electronic resource].
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Articles
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Main | ART VMJ V49 No2 7 (Browse shelf(Opens below)) | Available |
Includes references.
M. bovis cell extract antigens as filter-sterilized
lysozyme and sarkosyl extracts have been evalu-
ated and successfully used in vitro for diagnosis
of bovine tubcrculosis.They were compared with
the bovine PPD by measuring the cell mediated
immunity using the lymphocyte blastogenesis as-
Say (LBA) as detected by tetrazolism (MTI) re-
duction and skin sensitivity test, as well as meas-
uring the serum antibody levels using ELISA in
experimentally infected guinea pigs with M. bovis
and M. tuberculosis. The lymphocytic prolifera-
live response in the infected guinea pig groups 2
weeks post infection was higher than that ob-
tained 4 weeks post infection. The use of lyso-
Zyme and sarkosyl extract antigens was found to
be advantageous over the bovine PPD in lympho-
cytic blastogenesis assay due to their abilities to
confirm specificity and to discriminate between
animals infected with M. bovis and those infected
with M. tuberculosis.
Summary in Arabic
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