Conventional and molecular detection of Intramammary Staphylococcus Aureus infection in clinical and subclinical mastitis of buffaloes with a field therapeutic trial [electronic resource]
Language: English Summary language: Arabic Description: p. 31-43Other title:- الكشف التقليدي والجزيئي عن المكور العنقودي الذهبي داخل الضرع في حالات إلتهاب الضرع السريري والإكلينيكي للجاموس مع إجراء تجربة علاجية ميدانية [Added title page title]
- Assiut veterinary medical journal, 2019 v. 65 (160) [electronic resource].
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Main | ART AVMJ V65 No160 5 (Browse shelf(Opens below)) | Available |
Includes bibliographic reference.
Prevalence of Staphylococcus aureus mastitis in buffaloes was fundamentally aimed in the current work using culturing procedures and PCR Assay. Field therapeutic trial with follow-up for 10 consecutive weeks post therapy was carried out with monitoring of shedding pattern of Staphylococcus aureus. Three hundred and eighty buffaloes of different lactation seasons were examined in Sohag Governorate. Prevalence of clinically and subclinically infected buffaloes was 10.52% and 26.84%, respectively. Epizootiologically, prevalence of clinically and subclinically infected animals with mastitis in the 2nd, 3rd, and 4th lactation seasons was 12.00% and 32.00%; 20.83% and 41.67%; and 22.73% and 36.36%, respectively. Thereafter the trend of mastitis was significantly declined by increasing the numbers of lactation season. Over the 9th lactation season, there were no signs of mastitis. Bacteriologically, Staphylococcus aureus was the predominant pathogen followed by Streptococcus agalactiae and Escherichia coli. Staphylococcus aureus were sensitive to amoxicillin, enrofloxacin and ciprofloxacin but 50 % of the tested strains were resistant to penicillin and oxytetracycline. A Field therapeutic trial with synulox (Amoxicillin plus Clavulanic acid) with anti-inflammatory and supportive drugs was carried out in 10 diseased buffaloes (3 clinical and 7 subclinical). Three clinical and three subclinical forms were culled due to failure of therapy. Four cases of subclinical mastitis were apparently successfully treated. Shedding pattern of Staphylococcus aureus during the therapeutic period was weekly monitored and revealed that shedding was intermittent. In conclusion, Staphylococcus aureus is an outstanding notoriousness mastitogen and its early detection considers a crucial goal in control. PCR is a valuable test for detection of mastitogens than culture techniques but it is still precious practically.
Summary in Arabic
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