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Successful production of buffalo embryos from vitrified immature and mature oocytes in vitro [electronic resource].

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Abd-Allah, S. M

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Language: English Summary language: Arabic Description: p.332-343Other title:

إنتاج ناجح لاجنة الجاموس من بويضات غير ناضجة وناضجة مزججة بالتجميد السريع معمليا [Added title page title]

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Assiut veterinary medical journal, 2009 v. 55 (122) [electronic resource].

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In: Assiut Veterinary Medical Journal 2009.v.55(122)Summary: The current study was conducted to produce buffalo embryos in vitro from vitrified immature and mature oocytes. Cumulus oocytes romplexes (COCs) wm obtained from ovaries whil>h collel>ted from l! local abattoir Good quality COCs (immature oocytes) were vitrified immediately after collection. After 22-24 hours ofCOCs culture oocytes were vitrified similarly. All oocytes (immature and marure) were exposed to vitrification solution of different concentnltions (glycerol: 3.3,6.6 and 1000Ao) at 5, 5 and 10 minutes intervals respectively. The oocytes were finally placed in 10% glycerol and Immediately loaded in 0.25 ml plastic stnlws, each containing 100 )ul of 10% sucrose in Modified phosphate buffer saline (M-PBS).

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The current study was conducted to produce buffalo embryos in vitro from vitrified immature and mature oocytes. Cumulus oocytes romplexes (COCs) wm obtained from ovaries whil>h collel>ted from l! local abattoir Good quality COCs (immature oocytes) were vitrified immediately after collection. After 22-24 hours ofCOCs culture oocytes were vitrified similarly. All oocytes (immature and marure) were exposed to vitrification solution of different concentnltions (glycerol: 3.3,6.6 and 1000Ao) at 5, 5 and 10 minutes intervals respectively. The oocytes were finally placed in 10% glycerol and Immediately loaded in 0.25 ml plastic stnlws, each containing 100 )ul of 10% sucrose in Modified phosphate buffer saline (M-PBS).

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