Identification of cronobacter sakazakii isolated from powdered infant formula and stool of infants [electronic resource]

Includes bibliographic reference.

Cronobacter sakazakii is an emerging opportunistic pathogen contaminating powdered infant
formulas causing lethal threats to neonates and immune-deficient infants. It causes life
threatening infections, septicemia, neonatal meningitis, and necrotizing enterocolitis.
The aim of this study was to test the commercially available formulas that are intended for
consumption by 0-6 months old infants (neonates and immune-compromised infants), for the
presence of Cronobacter spp., and to determine the presence of C.sakazakii in the stool of
these infants who consumed these formulas through conventional methods as culturing,
biochemical tests and PCR. Fifty PIF samples (different brands) retailed in upper Egypt were
collected from Assiut University Children Hospital at the Gastroenterology and Hepatology
and Preterm Units, and we checked the presence of C.sakazakii in them. Fifty Stool samples
were also collected from the infants who were fed the studied PIF samples, to study the
presence of C.sakazakii in the stool of these infants. The samples underwent three steps of
pre-enrichment, enrichment procedures, and subculture onto chromogenic Enterobacter
sakazakii agar plates. Biochemical tests were afterwards carried out. Finally, molecular
characterization using specific PCR was done to detect Cronobacter sakazakii, targeting the
ESA_02797 gene which is found in all C.sakazakii strains. The results of this study shed light
on the immense need for applying effective prevention and control measures and taking all
the precautions needed during the production and preparation of PIF to hinder its
contamination with C.sakazakii and to prevent the spreading of such fatal infections to infants
with low immunity and neonates.
Keywords: C.sakazakii, PIF, Percentage, Detection, PCR

Summary in Arabic