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Rapid diagnosis of subclinical staphylococcus aureus-mastitis in dairy buffaloes using PCR technique [electronic resource].

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Mahmoued, M. M

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Language: English Summary language: Arabic Description: p.78-89Other title:

التشخيص السريع لالتهاب الضرع التحت السريري في الجاموس الحلاب الناتج عن عدوي المكور العنقودي الذهبى بأستخدام تقنية سلسلة التفاعل المتبلمر [Added title page title]

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Assiut veterinary medical journal, 2008 v. 54 (116) [electronic resource].

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In: Assiut Veterinary Medical Journal 2008.v.54(116)Summary: A total of 30 milking buffaloes, 3 to 5 years age, were randomly selected from farm of Faculty of Veterinary Medicine-Suez Canal University for investigation of Staphylococcus aureus subclinical mastitis by California mastitis test (CMT), culture method and Polymerase Chain Reaction (PCR). The detected cases were isolated and a controlling attempt was carried out prior and post milking processes. The total number of quarters affected by subclinical mastitis using CMT w.as 30 (29.41%) representing 18 buffaloes (60%). PCR is rapid technique for detection of Staphylococcal subclinical mastitis when compared with culture methods.

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A total of 30 milking buffaloes, 3 to 5 years age, were randomly selected from farm of Faculty of Veterinary Medicine-Suez Canal University for investigation of Staphylococcus aureus subclinical mastitis by California mastitis test (CMT), culture method and Polymerase Chain Reaction (PCR). The detected cases were isolated and a controlling attempt was carried out prior and post milking processes. The total number of quarters affected by subclinical mastitis using CMT w.as 30 (29.41%) representing 18 buffaloes (60%). PCR is rapid technique for detection of Staphylococcal subclinical mastitis when compared with culture methods.

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