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Employment of the hypo-osmotic swelling test to evaluate functional integrity of the washed buffalo spermatozoal membrane [electronic resource].

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EL-Haralry, M.A

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Language: English Summary language: Arabic Description: p.14-30Other title:

إستخدام إختبار إنخفاض الأسموزيه للتقييم الوظيفي للغشاء الخلوي في الحيوانات المنوية منزوعة البلازما في الجاموس المصري [Added title page title]

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Assiut veterinary medical journal, 2006 v. 52 (109) [electronic resource].

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In: Assiut Veterinary Medical Journal 2006.v.52(109)Summary: Twelve buffalo bulls of 3-5 years of age and 400 to 500 kg live body weights were used in the present study. The experimental work was carried out to establish the optimum condition of osmotic pressure using lactose-Na-citrate solution at different osmolarities (50, 100, 150, 200, and 300 mOsmol/ L) either washed or non-washed buffalo spermatozoa. The percentage of sperm motility, grade of the progressive sperm motility (score), percentages of spermatozoa with coiled tail, and spermatozoa swelling, were determined during incubation at 37 °C for 0, 5, 15,30 and 60 minutes.

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Twelve buffalo bulls of 3-5 years of age and 400 to 500 kg live body weights were used in the present study. The experimental work was carried out to establish the optimum condition of osmotic pressure using lactose-Na-citrate solution at different osmolarities (50, 100, 150, 200, and 300 mOsmol/ L) either washed or non-washed buffalo spermatozoa. The percentage of sperm motility, grade of the progressive sperm motility (score), percentages of spermatozoa with coiled tail, and spermatozoa swelling, were determined during incubation at 37 °C for 0, 5, 15,30 and 60 minutes.

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