Effect of trehalose,cysteine and hypotaurine on buffalo bull sperm freezability,ultrastructure changes and fertilizing Potentials [electronic resource].

Includes references.

Cryopreservation induces sublethal damage to the spermatozoa, which leads to reduce their fertile life. The objective of the present study was to investigate the effect of trehalose, cysteine and hypotaurine on freezability, ultrastructure and in vitro fertilizing potentials of the buffalo spermatozoa. Buffalo spermatozoa were cryopresreved with Tris egg yolk extender containing 7% glycerol suplemmented with 1OO mM trehalose, 1OO mM trehalose+ 5 mM cysteine, 1OO mM trehalose+ 25 mM hypotaurine, 100 mM trehalose + 5 mM cysteine + 25 mM hypotaurine or Trisbased extender only (control). Cryopresreved spermatozoa were assessed for post- thawing sperm motility; viability and acrosomal integrity, ultrastructure changes, biochemical activity and in vitro fertilizing potentials. The current results clearly indicated that adding a mixture of 100 mM trehalose , 5 mM cysteine and 25 mM hypotaurine to Tris exteriaer significantly improved (P<0.05) post-thawing sperm motility, viability index and maintained acrosomal integrity following cryopreservation (63.33±7.27%, I55.83±21.06 and I2.33±2.73%, respectively) compared with the control spermatozoa (38.33±4.41%, 94.I7±12.28 and 25.33±3.49%, respectively).

Summary in Arabic.