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Isolation of clostridium species from geese and their susceptbility to antibacterial agents in Assiut governorate [electronic resource].

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Abdel-Rahman, A.A

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Language: English Summary language: Arabic Description: p.264-276Other title:

عزل ميكروبات الكلوستريديم من الاوز وتأثير المضادات الحيوية عليه بمحافظة أسيوط [Added title page title]

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Assiut veterinary medical journal, 2007 v. 53 (113) [electronic resource].

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In: Assiut Veterinary Medical Journal 2007.v.53(113)Summary: A total of 200 geese (20 apparently healthy, 120 diarrhotic geese and 60 freshly dead) collected from privately owned at Assiut province were subjected to post-mortem and bacteriological examination for the prevalence of clostridia microorganisms in geese. There are variation in the prevalence rate of clostridium species isolated from apparently healthy geese which lower than ofdiarrhotic geese and freshly dead & slaughtered. It was found 6 (30%) in apparent healthy, 80 (66.7%) in diseased geese, while was 44 (73.3%) in dead geese According to morphological characters and biochemical reactions, 130 clostridium isolates were successfully isolated with an incidence of 65%. The most important isolates was C. perfrigens with incidence of 74 (56.9%) followed by C.sporogenes was 26 (20.7%), C.colinum was 19 (14.6%) and C. sordelli was II (8.5%). For the typing of C perfrigens isolated, type "A" was the most prevalent with incidence of (72.3%) followed by type "C" with incidence of (18.5%) and type "D: with incidence of (9.3%). Towage groups of geese (15 and 45-day old) were used to test the pathogenicity of C.perfringens type "A", C. sporogenes, C.colinum and C. sordelli. The mortality in geese at 15-day old through oral administration of C.perfringens type "A", C. sporogenes, C.colinum and C. sordelli were 60%, 40%, 20% and 20% respectively while were 100%, 80%, 60% and 20% respectively through I/M inoculation and the mortality rate in geese at 45-day old through oral administration were 40%, 20%, 0.0% and 0.0% while were 80%, 40%, 20% and 0.0% respectively through I/M inoculation. Sensitivity test of Clostridia strain isolates against some antibiotics in vitro showed that, Penicillin, Ampcillin, Amoxicillin, and Chloramephenical were highly sensitivity, while Lincomycin, Norfloxacin and Kanamycin were moderate and were resistant to Streptomycin, Gentamycin Tetracycline and Nalidixic acid.

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A total of 200 geese (20 apparently healthy, 120 diarrhotic geese and 60 freshly dead) collected from privately owned at Assiut province were subjected to post-mortem and bacteriological examination for the prevalence of clostridia microorganisms in geese. There are variation in the prevalence rate of clostridium species isolated from apparently healthy geese which lower than ofdiarrhotic geese and freshly dead & slaughtered. It was found 6 (30%) in apparent healthy, 80 (66.7%) in diseased geese, while was 44 (73.3%) in dead geese According to morphological characters and biochemical reactions, 130 clostridium isolates were successfully isolated with an incidence of 65%. The most important isolates was C. perfrigens with incidence of 74 (56.9%) followed by C.sporogenes was 26 (20.7%), C.colinum was 19 (14.6%) and C. sordelli was II (8.5%). For the typing of C perfrigens isolated, type "A" was the most prevalent with incidence of (72.3%) followed by type "C" with incidence of (18.5%) and type "D: with incidence of (9.3%). Towage groups of geese (15 and 45-day old) were used to test the pathogenicity of C.perfringens type "A", C. sporogenes, C.colinum and C. sordelli. The mortality in geese at 15-day old through oral administration of C.perfringens type "A", C. sporogenes, C.colinum and C. sordelli were 60%, 40%, 20% and 20% respectively while were 100%, 80%, 60% and 20% respectively through I/M inoculation and the mortality rate in geese at 45-day old through oral administration were 40%, 20%, 0.0% and 0.0% while were 80%, 40%, 20% and 0.0% respectively through I/M inoculation. Sensitivity test of Clostridia strain isolates against some antibiotics in vitro showed that, Penicillin, Ampcillin, Amoxicillin, and Chloramephenical were highly sensitivity, while Lincomycin, Norfloxacin and Kanamycin were moderate and were resistant to Streptomycin, Gentamycin Tetracycline and Nalidixic acid.

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