Investigation of certain proinflammatory and antiinflammatory cytokine levels in the gut biopsies of cats with inflammatory bowel disease using realtime PCR [electronic resource].

Includes reference.

The aim of this study was to measure and quantify levels of some cytokines implicated in the irnmunopathogenesis in the gut biopsies of cats with inflanunatory bowel disease (lBD) using real time polymerase chain reaction (PCR). Frozen duodenal biopsies were collected from cats referred for investigation of chronic vomiting andlor diarrhea (n=3) that were diagnosed with IBD. Duodenal biopsies from control cats (n=5) were examined for the expression of mRNA for pro-inflammatory cytokines IFN-y, IL-12 and 1NF-a, and anti-inflammatory cytokines IL4 and IL-I0 using real-time RT-PCR. There was no significant difference between levels of expression of different tested mRNA cytokines in the two groups of cats (p>O.OS). These findings suggest that the immunopathogenesis of feline IBD involve different mechanisms from that of other studied species and does not follow the classic Th1/Th2 paradigm.