Genetic diversity of u. betae, the cause of sugar beet rust in Egypt [electronic resource].

Includes references.

Sodium Dodecyle Sulphate (SDS) Protein Electrophoresis and Random Amplified Polymorphic DNA (RAPD-PCR) were used to distinguish between Uromyces betae Tul. ex Kick isolates collected from different locations of Egypt during 2005/ 06. Twenty three isolates were used in this study. Total protein electrophoresis analysis exhibited 85.71 % polymorphism among the studied isolates. Dendrogram analysis separated the studied isolates into two groups. There were isolate-specific bands by which isolates, particularly those belonged to different groups, could be identified. There was no correlation between clustering in the protein dendrogram and geographic origin of the tested isolates. RAPD analysis was carried out using seven arbitrary primers, in a preliminary study, primer OP2 showed 100 % polymorphism among the twenty three tested isolates. Five bands were recorded as isolate specific bands. Dendrogram analysis separated the studied isolates into three groups. Depending on the obtained results from phylogenetic relationships study among the twenty three studied isolates by SDS-protein electrophoresis and OP2 arbitrary primer, fourteen isolates out of the twenty three tested isolates were selected and subjected to RAPD-PCR study using six arbitrary primers to estimate polymorphism among them in a complementary study. The complied date for the six primers recorded 98.33 % polymorphism among the fourteen isolates.

Summary in Arabic.

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