Central composite design for the optimization of Plackett-Burman design18B-glycyrrhetinic acid production by trichosporon jirovecii [electronic resource].

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In our previous study Plackett-Burman design was used to select the most effective variables for a high production of 18B-glycyrrhetinic acid (GA). In this work the Central Composite Design (CCD) combined with Response Surface Methodology (RSM) were used to optimize the conditions for GA production from glycyrrhizin (GL) by the yeast Trichosporon jirovecii. The used independent variables were initial pH, incubation time, glucose, yeast extract and com steep liquor. A mathematical model that is able to predict the effect of independent variables towards the responses was established by multiple regression analysis. Analysis of variance was used to determine the adequacy between the model and experimental value. The results indicated that the model was semi-adequate with satisfactory of the RSquare (O.54B). It was fairly good experimental model fds the actual data. Using this design a high cone. (15B mg)of GA was produced from 0.6 g of crude GL

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