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Use of hypoosmotic swelling test for evaluating camel spermatozoal membrane integrity in relation to different solutions [electronic resource].

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Zeidan, A.E.B

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Language: English Summary language: Arabic Description: P.109-122Other title:

استخدام اختبار انخفاض الأسموزية لتقييم سلامة الغشاء الخلوى فى الحيونات المنوية للابل تحت تأثير المحاليل المختلفة.‪ [Added title page title]

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Veterinary medical journal , 2008 v.56 (1) [electronic resource].

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In: Veterinary Medical Journal 2008.v.56(1)Summary: Five male camels at 5 to 10 years of age and 400 - 600 kg body weight, were used in the present study.Semen was collected, evaluated, pooled and extended with seven different hypoosmotic solutions (glucose - yolk-citrate: GYC, fructose-yolkcitrate FYC, lactose-yolk-citrate: LYC, sucroseyolk- citrate: SYC, tris-yolk-fructose: TYF, skimcow- milk: SCM and skim camel-milk: SLM) at a level of 100 mOsmol/L and then incubated at 37°C for up to 60 minutes. After each incubation time (0, 5, 15,30 and 60 minutes), the percentages of sperm motility, spermatozoa with intact acrosome, spermatozoa with coiled tails and swollen spermatozoa, were estimated.

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Five male camels at 5 to 10 years of age and 400 - 600 kg body weight, were used in the present study.Semen was collected, evaluated, pooled and extended with seven different hypoosmotic solutions (glucose - yolk-citrate: GYC, fructose-yolkcitrate FYC, lactose-yolk-citrate: LYC, sucroseyolk- citrate: SYC, tris-yolk-fructose: TYF, skimcow- milk: SCM and skim camel-milk: SLM) at a level of 100 mOsmol/L and then incubated at 37°C for up to 60 minutes. After each incubation time (0, 5, 15,30 and 60 minutes), the percentages of sperm motility, spermatozoa with intact acrosome, spermatozoa with coiled tails and swollen spermatozoa, were estimated.

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