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Molecular analysis of seven introductions fodder beet [electronic resource].

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Abd El-Ghawad, M. F

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Language: English Summary language: Arabic Description: p.203-227Other title:

التحليل الجزيئى لسبعة مستوردات من بنجر العلف [Added title page title]

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Egyptian journal of agricultural research, 2010 v. 88 (1), Special issue [electronic resource]:

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In: Egyptian Journal of Agricultural Research 2010.v.88(1)SPSummary: The fodder beet does not set flower in Egypt. Attempts have been done to induce flowering in fodder beet, but they have not been successful due to factors of infertility. So, seeds are importing from different countries such as France, Bulgaria and Hungary. The identity of germplasm resources of fodder beet would be a great value for breeding. Due to the floral biology of fodder beet varieties and hybrids, the expected genetic variability is high. lsozymes (peroxidase and Esterase), seed protein. and RAPD (five primers) markers were used in this study. It was observed that Betarose and 5tarmoon have the highest values of germination of the seven varieties at 4 days and at 10 days. Results revealed that the genetic similarity matrix ranged from 68.6% to 93.3% for total isozyme, 61.5% to 94.1% for seed protein, 60.4% to 80.4% for RAPDs. The overall similarity matrix revealed that the highest similarity was (82.8%), which occurred between Rota and Jary followed by Jamon and Starmoon (81.4%) and Rota and Jamon (80.0%), while the lowest similarity was (67.5%) between Vroushanger and Monro followed by (68.9%) Vroushanger and Rota. The relative information value of each marker can be evaluated on the basis of its polymorphic information content (PIC), which ranged from 3.6 to 29%, while general mean was equal 21.3% with 2 number of alleles in every system. Heterozygosity ranged from 0.04 in l3-esterase to 0.48 in OP-A19 followed by OP-GOS 0.47.

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The fodder beet does not set flower in Egypt. Attempts have been done to induce flowering in fodder beet, but they have not been successful due to factors of infertility. So, seeds are importing from different countries such as France, Bulgaria and Hungary. The identity of germplasm resources of fodder beet would be a great value for breeding. Due to the floral biology of fodder beet varieties and hybrids, the expected genetic variability is high. lsozymes (peroxidase and Esterase), seed protein. and RAPD (five primers) markers were used in this study. It was observed that Betarose and 5tarmoon have the highest values of germination of the seven varieties at 4 days and at 10 days. Results revealed that the genetic similarity matrix ranged from 68.6% to 93.3% for total isozyme, 61.5% to 94.1% for seed protein, 60.4% to 80.4% for RAPDs. The overall similarity matrix revealed that the highest similarity was (82.8%), which occurred between Rota and Jary followed by Jamon and Starmoon (81.4%) and Rota and Jamon (80.0%), while the lowest similarity was (67.5%) between Vroushanger and Monro followed by (68.9%) Vroushanger and Rota. The relative information value of each marker can be evaluated on the basis of its polymorphic information content (PIC), which ranged from 3.6 to 29%, while general mean was equal 21.3% with 2 number of alleles in every system. Heterozygosity ranged from 0.04 in l3-esterase to 0.48 in OP-A19 followed by OP-GOS 0.47.

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