Normal view MARC view ISBD view

Use of random amplifide polymorphic DNA (rapd) to differentiate among isolates of Fusarium spp. pathogenic on cotton [electronic resource].

By:

EI-Samawaty, A.M.A

Contributor(s):

Language: English Summary language: Arabic Description: p.811-827Other title:

أستخدام التضاعف العشوائي لمناطق متابينة من الحمض النووي DNA للتفرقة بين عزلات أنواع الفيوزاريوم الممرضة للقطن.‪ [Added title page title]

Uniform titles:

Journal of biological chemistry and environmental sciences, 2008 v. 3 (1) Part IV [electronic resource].

Subject(s):

Online resources:

Full Text Article.

In: Journal of Biological Chemistry and Environmental Sciences 2008.v.3(1)IVSummary: Twenty Isolates of nine Fusarium spp. were used in this study they were obtained from roots of cotton seedlings infected with damping off disease. Pre-emergence damping-off was recorded 15 days after planting for the cotton cultivars of Giza 80, and Giza 90. Postemergence damping-off, survival and dry weight (mg/plant) were recorded 45 days after planting. Isolate, cultivar, and isolate X cultivar interaction were significant sources of variation in all the tested parameters except the cultivar which was a nonsignificant source of variation in pre-emergence damping-off. Random Amplified Polymorphic DNA (RAPD) Technique has been used as a molecular technique for typing and genetic characterization of Fusarium spp. using three different decamer random primers (3, 4, and 5). DNA was extracted from 9 different Fusarium spp. and used for polymerase chain reaction (PCR). The similarity levels were determined by Cluster analysis for RAPD-PCR profiles via phylogeny tree. Primer 3 was successful in separating the isolates of F. solani, F. moniliforme, F. oxysporum, and F. semitectum. Isolates of F. oxysporum gave high DNA similarity (99.65%) with this primer. Primer 4 was successful in the separation of F. solani, F. oxysporum, F. moniliforme, F. semitectum, and F. sporotrichioides. This primer is suggested to be the best of all primers in separating F. sporotrichioides since it showed the highest DNA similarity degree (98.87%). Primer 5 was successful only in the separation of F. solani, F. oxysporum, F. moniliforme, and F. semitectum isolates. This primer was the best of all the primers in the separation of F. solani, and F. semitectum since it showed high DNA similarity degrees among these isolates (99.71 %, and 99.78% respectively). The results in this study evoke that isolates of Fusarium spp. exhibited diversity in pathogenicity on cotton cultivar and demonstrate that the use of RAPD analysis is useful in classification of Fusarium spp.

Average rating: 0.0 (0 votes)

No physical items for this record

Includes reference.

Twenty Isolates of nine Fusarium spp. were used in this study they were obtained from roots of cotton seedlings infected with damping off disease. Pre-emergence damping-off was recorded 15 days after planting for the cotton cultivars of Giza 80, and Giza 90. Postemergence damping-off, survival and dry weight (mg/plant) were recorded 45 days after planting. Isolate, cultivar, and isolate X cultivar interaction were significant sources of variation in all the tested parameters except the cultivar which was a nonsignificant source of variation in pre-emergence damping-off. Random Amplified Polymorphic DNA (RAPD) Technique has been used as a molecular technique for typing and genetic characterization of Fusarium spp. using three different decamer random primers (3, 4, and 5). DNA was extracted from 9 different Fusarium spp. and used for polymerase chain reaction (PCR). The similarity levels were determined by Cluster analysis for RAPD-PCR profiles via phylogeny tree. Primer 3 was successful in separating the isolates of F. solani, F. moniliforme, F. oxysporum, and F. semitectum. Isolates of F. oxysporum gave high DNA similarity (99.65%) with this primer. Primer 4 was successful in the separation of F. solani, F. oxysporum, F. moniliforme, F. semitectum, and F. sporotrichioides. This primer is suggested to be the best of all primers in separating F. sporotrichioides since it showed the highest DNA similarity degree (98.87%). Primer 5 was successful only in the separation of F. solani, F. oxysporum, F. moniliforme, and F. semitectum isolates. This primer was the best of all the primers in the separation of F. solani, and F. semitectum since it showed high DNA similarity degrees among these isolates (99.71 %, and 99.78% respectively). The results in this study evoke that isolates of Fusarium spp. exhibited diversity in pathogenicity on cotton cultivar and demonstrate that the use of RAPD analysis is useful in classification of Fusarium spp.

Summary in Arabic.

There are no comments on this title.

to post a comment.