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Improving detection means for strawberry gray mould caused by Botrytis cinerea in Egypt [electronic resource].

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Abdel Wahab, Hala

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Language: English Summary language: Arabic Description: p.107-119Other title:

تحسين وسائل الكشف عن مرض العفن الرمادي في الفراولة المتسبب عن فطر Botrytis cinerea في مصر.‪ [Added title page title]

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Egyptian journal of phytopathology, 2010 v. 38 (1-2) [electronic resource].

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In: Egyptian Journal of Phytopathology 2010.v. 38(1-2)Summary: Botrytis cinerea is an economically important patbogen. Twenty seven B. cinerea isolates were cbaractcrizcd for their pathogenicity on strawberry fruits. A cheap and simple method was developed for the identification of Botrytis cinerea isolates, the cause of gray mould, using modified Kerssies' selective medium (mKERS). The other fungal species could not grow on this medium Moreover, there was no relation between the aggressiveness of each tested isolate of B. cinerea and its radial growth level on mKERS. The results showed that the 27 isolates of B. cinerea grew well and produced a dark brown pigment on mKERS. These results indicate that this simple method could be used for the detection of gay mould. Additionally, genetic diversity was investigated using PCR assay. The Egyptian population structure of B. cinerea was investigated by detecting the existence of two. transposable elements (TEs): Flipper and Boty in the genome. Among 12 isolates tested, 10 have only one transposable element, Boty element, whereas 2 have neither of these elements (vacuma type). This result. is useful for identifYing and classifying the Egyptian population of B. cinerea. especially for the isolates exhibited high virulence according to their genomic structure.

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Botrytis cinerea is an economically important patbogen. Twenty seven B. cinerea isolates were cbaractcrizcd for their pathogenicity on strawberry fruits. A cheap and simple method was developed for the identification of Botrytis cinerea isolates, the cause of gray mould, using modified Kerssies' selective medium (mKERS). The other fungal species could not grow on this medium Moreover, there was no relation between the aggressiveness of each tested isolate of B. cinerea and its radial growth level on mKERS. The results showed that the 27 isolates of B. cinerea grew well and produced a dark brown pigment on mKERS. These results indicate that this simple method could be used for the detection of gay mould. Additionally, genetic diversity was investigated using PCR assay. The Egyptian population structure of B. cinerea was investigated by detecting the existence of two. transposable elements (TEs): Flipper and Boty in the genome. Among 12 isolates tested, 10 have only one transposable element, Boty element, whereas 2 have neither of these elements (vacuma type). This result. is useful for identifYing and classifying the Egyptian population of B. cinerea. especially for the isolates exhibited high virulence according to their genomic structure.

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