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Bulked segregant analysis for developing genetic markers of drought tolerance in faba bean (Vicia faba L.) [electronic resource]

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Afiah, S. A

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Language: English Summary language: Arabic Description: p.89-112Other title:

تحليل ضم الانعزالات المتفارقة لاستنباط كاشفات وراثية لتحمل الجفاف في الفول [Added title page title]

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Egyptian journal of desert research, 2007 v.57 (1) [electronic resource].

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In: Egyptian Journal of Desert Research 2007.v.57(1)Summary: A field experiment was conducted to study the response of two contrasting drought tolerant parents (L8 and L3), their F₁, F₂ and two comparative varieties (G716 and G461) to cultivation under natural rain conditions at Maryout region. Seed yield/plant and its components revealed significant differences among all genotypes for all traits recorded. The F₂ tolerant segregants group surpassed all other genotypes tested for seed yield/plant. This is attributed mainly to number of pods/plant and partially to seed index. The results of protein markers indicated that parental drought tolerant line and F₂ tolerant segregant group had a specific band of 87KDa molecular weight which could be used to distinguish the drought tolerant genotypes among others, for instance F₁ cross (L3xL8) and Giza 461 gave the same band. Acid phosphatase isozyme patterns didn't give clear cut markers for the discrimination between drought tolerant and drought sensitive genotypes. A positive band for drought sensitive genotypes as it was observed in F₂S and L8 sensitive parent. β-esterase (band No.6) and β-esterase (band No.5) isozyme patterns could be used to distinguish the drought sensitive genotypes among others.

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A field experiment was conducted to study the response of two contrasting drought tolerant parents (L8 and L3), their F₁, F₂ and two comparative varieties (G716 and G461) to cultivation under natural rain conditions at Maryout region. Seed yield/plant and its components revealed significant differences among all genotypes for all traits recorded. The F₂ tolerant segregants group surpassed all other genotypes tested for seed yield/plant. This is attributed mainly to number of pods/plant and partially to seed index. The results of protein markers indicated that parental drought tolerant line and F₂ tolerant segregant group had a specific band of 87KDa molecular weight which could be used to distinguish the drought tolerant genotypes among others, for instance F₁ cross (L3xL8) and Giza 461 gave the same band. Acid phosphatase isozyme patterns didn't give clear cut markers for the discrimination between drought tolerant and drought sensitive genotypes. A positive band for drought sensitive genotypes as it was observed in F₂S and L8 sensitive parent. β-esterase (band No.6) and β-esterase (band No.5) isozyme patterns could be used to distinguish the drought sensitive genotypes among others.

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